Teucrium polium L: An updated review of phytochemicals and biological activities

Objective: Medicinal plants and their components are potential novel sources for developing drugs against various diseases. Teucrium polium L. (syn Teucrium capitatum L. or felty germander) from the Lamiaceae family, is widely distributed in the dry and stony places of the hills and deserts of almost all Mediterranean countries, southwestern Asia, Europe, and North Africa. Based on traditional Iranian medicine (TIM), T. polium is used for treating many diseases, including abdominal pain, indigestion, and type 2 diabetes. Materials and Methods: In our previous review article published in 2012 and based on 100 articles published from 1970 to 2010, the main compounds purified from T. polium were terpenes, terpenoids, and flavonoids with antioxidant, anticancer, anti-inflammatory, hypoglycemic, hepatoprotective, hypolipidemic, antibacterial, and antifungal activities. Results: In this article, the phytochemistry and pharmacological activities of the plant reported from 2011 to 2020 have been evaluated. Therefore, a search was done in the databases PubMed, Science Direct and Google Scholar, Scopus, and Web of Science with the terms "T. polium," "T. capitatum." and felty germander’, which included about 100 articles published since 2011 about T. polium pharmacological activities and isolated compounds. Most studies of this review focused on the antioxidant and antidiabetic effects of the plant Conclusion: Considering the position of T. polium in folk medicine, mainly as an antidiabetic agent, purification, structural and biological characterization of the active components appears essential for effective use of the plant.


Introduction
Traditional systems of medicine provide valuable information on natural remedies. Medicinal plants, as important sources of new chemicals with potential therapeutic effects, play a significant role in discovering new drug leads. As one of the largest and most distinguished families of flowering plants, Lamiaceae has 236 genera and 6900-7200 species worldwide, with a wide range of biological activity and diverse phytochemicals (Naghibi et al., 2005;Raja, 2012). Several experimental studies on species of this family confirmed the effectiveness of some of its traditional applications. Teucrium polium which belongs to the family Lamiaceae, is a perennial shrub, 20-50 cm high, and it is widely distributed in the dry and stony places of the hills and deserts of almost all Mediterranean countries, southwestern Asia, Europe, and North Africa. Sessile, oblong, or linear leaves with a length of about 3 cm (Table  1) (Feinbrun-Dothan, 1978;Yazdanparast and Bahramikia, 2012). T. polium contains several subspecies and varieties, including aragonense, aurasiacum, capitatum cylindricum, expansum, gnaphalodes, pilosum, polium, vincentinum, yalentinum and many others ( Figure 1) (El Oualidi et al., 1999). Traditionally, in the Mediterranean countries, T. polium has been used against various types of pathological conditions, such as gastrointestinal disorders, inflammations, diabetes and rheumatism (Abdollahi et al., 2003;Tariq et al., 1989). It is also used as an antibacterial, antiulcer, hypotensive, antispasmodic, anorexigenic and antipyretic agent (Autore et al., 1984;Suleiman et al., 1988;Gharaibeh et al., 1989). This plant is abundantly found in Iran and names Kalporeh. In traditional Iranian medicine (TIM), its tea is used for treating many diseases such as abdominal pain, indigestion, common cold, and urogenital diseases (Abdollahi et al., 2003). In our previous article in 2012, we mentioned many compounds mainly belonging to terpenes, terpenoids and flavonoids for which, pharmacological activities such as antioxidant, anticancer, anti-inflammatory, hypoglycemic, hepatoprotective, hypolipidemic, antibacterial and antifungal properties were reported (Bahramikia and Yazdanparast, 2012). In this article, we extend our attention to the phytochemical and pharmacological activities of the plant and its characterized constituents reported between the years 2011-2020.  A-winter (late January); Bspring/summer (late May/early July); C-summer (late July/middle August); D-autumn (late November).

Materials and Methods
In our previous review article published in 2012 based on 100 articles published from 1970 to 2010. In this article, the phytochemistry and pharmacological activities of the plant from 2011 to 2020 have been evaluated. Therefore, a search was done in the databases PubMed, Science Direct and Google Scholar, Scopus, and Web of Science with the terms "T. polium," "T. capitatum." and felty germander', which included about 100 articles published since 2011 about T. polium pharmacological activities and isolated compounds.

Ethnobotanical studies and traditional medicine use
In different parts of the world, different parts of this plant are used in various forms, alone or combined with other plants in traditional medicine. It has been reported that a cup of infusion of the leaves of T. polium after a meal is recommended because of its antidiarrheal, hypnotic, antiparasitic, antifungal, and antitussive actions. Also, the usage is recommended to treat diabetes mellitus, rheumatoid arthritis, paranasal sinusitis, bloating, menorrheal discharge, wound disinfection, gingivitis, tonsillitis, acne, itching, dyspepsia, and amenorrhea (Miikaili et al., 2012). T. polium L. is traditionally used in the town of Elazığ, Turkey, for high cholesterol, cold, and flu. T. polium L. extract was shown to induce hypoglycemic, antipyretic, and intestinal motility activities. The leaves are used for diabetes, kidney and liver diseases, stomach and intestinal pain, diabetes and hemorrhoids Hayta et al. (2014). The results of a research conducted in Edremit Bay showed that T. polium was extensively used for commercial purposes. Infusion of flowering and branches of T. polium L. is used in traditional medicine to treat diabetes and kidney stones (O.Ad. (Oral administration) by drinking one teacup two times a day for one week (Polat and Satıl, 2012). In the markets of Mashhad, Iran, T. polium are used by the traditional medicine for antacid, indigestion, diabetes, treatment of colic and diarrhea (Amiri and Joharchi, 2013). Reports indicated that aqueous extract of T. polium with tail flick test showed antinociceptive activity. It's possible mechanism is inhibiting release of acid arachidonic and synthesis of prostaglandins and effect on opioid system binding to pain receptors, affecting ligandsensitive channels and decreasing sodium entrance (Bahmani et al., 2014). In another study, Nasab and Khosravi (2014) reported that fumes from burning Descurainia sophia seeds combined with T. polium flowers heal earaches and ear infections. Also, flower extract / plant powder of T. polium are used to treat chickenpox, ear infections, ear pain, abdominal ache, diarrhea in infants, acne and skin blemishes. Sadeghi et al. (2014) reported that flowers and branches of T. polium L. used in traditional medicine to treat antipyretic, insect, snake, and scorpionbite; also, it is used for wound healing, stomach ache, abdominal pain, flatulency, emesis, stomach acidification, hypertension, toothache, diabetes, and hyperlipidemia and administered as a sedative. Ethnopharmacological use of them was reported for gastrointestinal disorders, common cold, and fever, and as an antioxidant, antispasmodic, hypoglycaemic, and anti-inflammatory agent (Eissa et al., 2014). Two years later, Ali-Shtayeh et al. (2016) reported that aerial part of T. polium in ethno-veterinary is used to treat diarrhea, colic, bleeding, scabies, and flatulence. T. polium as a medical treatment in Bordj Bou Arerridj region, Northeast Algeria, is used as infusion and powder to treat diabetes and migraine. Ouelbani et al. (2016) reported that this plant is used in traditional medicine for the treatment of wounds, coagulants, chills, fever, and digestive system problems. Also, it is used for treatment of wound, hemorrhoids, weakness, chills, fever, and pinworms and as an anti-inflammatory, astringent, detergent, febrifuge (paludisme), antihyperglycemia, disinfectant, stomachic, hypotensive, coagulant, vermifuge, and antidiabetic agen (Miara et al., 2019).

Biological activities
In our previous article in 2012, based on 100 articles published from 1970 to 2010, several pharmacological activities, including antioxidant, anticancer, antiinflammatory, hypoglycemic, hepatoprotective, hypolipidemic, antibacterial, and antifungal effect of different extracts and compounds isolated from T. polium were reported. In this study, all the biological effects of the plant reported from 2011 to 2020 are shown (Table  4).

Antioxidant activities
Different studies have shown that overproduction of free radicals and oxidative stress cause various diseases, including cancer, cataracts, cardiovascular disease, immune system decline, and brain dysfunction. Thus, using plants such as T. polium, which are rich in antioxidant compounds, is an important strategy against these diseases (Hemmati Hassan Gavyar and Amiri, 2018). In a review article by Krishnaiah et al. (2011), several compounds including rutin, apigenin, 3', 6dimethoxy apigenin, and 4', 7dimethoxyapigenin from the methanolic solvent of T. polium (aerial parts) were reported as the main components with high antioxidant ability as assessed by 2, 2diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and βcarotene bleaching assays ( IC 50 =20.1±1.7 µg/ml and 25.8±1.2 mm, respectively). Goulas et al. (2012), using High performance liquid chromatography-solid phase extractionnuclear magnetic resonance )HPLC-SPE-NMR( and HPLC-DPPH techniques, indicated that phenylpropanoid glycosides purified from the polar extracts of T. Polium are responsible for antioxidant activity (66-80%). Different methods examined the effects of extraction by various solvents on the antioxidant activity of leaves of T. polium. The results showed that among the various extracts, the nbutanol extract has higher antioxidant power, likely due to the presence of compounds such as flavonoids, iridoids, and phenylethanoids (De Marino et al., 2012). In another study, antioxidant activities of methanolic extracts of some Lamiaceae species, including T. polium, were studied by Khaled-Khodja et al. (2014). Their research showed that among examined plants, Mentha pulegium and T. polium had high antioxidant activity. Also, T. polium had the highest total phenolics and total flavonoids contents compared to other plants. Antioxidant activities of the ethanolic extracts of selected Lamiaceae species, including T. polium, were investigated by Vladimir-Knežević et al., 2014. Their findings showed that T. polium extract is a powerful antioxidant (IC 50 =5.90±0.12 µg/ml). Verbascoside, poliumoside, jaranol, and isorhoifolin were the four compounds isolated from T. polium var. gnaphalodes; Jaranol showed the highest tyrosinase inhibitory activity, and poliumoside had the highest antioxidant activity as assessed by fluorescence recovery after photobleaching )FRAP ( (14.32 mmol/g) and DPPH radical scavenging ) IC 50 = 0.042 µg/ml) assays compared to other compounds (Boghrati et al., 2016). Sayyad and Farahmandfar (2017) reported that essential oil of T. polium can be useful to oxidative stability of the canola oils. Rahmouni et al. (2017) reported that T. polium has protective effect on hematological and some biochemical parameters against carbon tetrachloride (CCl4) induced toxicity in rats. In another study, antioxidant activity and total phenolic and flavonoid contents of methanol, ethanol, water, and ethyl acetate extracts of T. polium were investigated by El Atki et al. (2019) who showed that in both methods (DPPH radical scavenging and FRAP), the methanolic extract had the highest antioxidant activity (lowest IC 50 ). Also, the total phenolic and flavonoid contents of this extract were higher than water, and ethyl acetate extracts. In addition, the highest total antioxidant capacity was related to water extract. El Atki et al.
(2020) identified that the essential oils of T. polium subsp. aurum have a higher antioxidant power than T. polium subsp. polium, in both methods DPPH radical scavenging and FRAP. In addition, results showed that in the total antioxidant capacity method, T. polium subsp. aureum had a significant activity (3308.27 mg equivalent to ascorbic acid/g of EO). Recently, it was reported that T. polium extract could be used as a natural antioxidant for the stability and safety of canola oil during frying. Tocopherols and phenolic compounds in the extract are probably responsible for this feature (Asadi and Farahmandfar, 2020).

Antidiabetic activities
T. polium has been long recommended in Iranian folk medicine for its antidiabetic activities. In a study by Kasabri et al. (2011), in vivo and in vitro, antihyperglycemic effects of five selected indigenous plants from Jordan used in traditional medicine were investigated. In vitro model results demonstrated that T. polium did not have appreciable antiamylase or anti-glucosidase effectiveness.
In addition, T. polium aqueous extracts did not evoke any substantial reduction in the overall glycemic excursion in the treated animals. Each T. polium-supplemented group had a significant decrease (p<0.05) in cornstarch-induced acute hyperglycemia 45 min post intragastric starch administration. They concluded that other modes of action could explain their substantial antihyperglycemic activities in starch-treated rats (Kasabri et al., 2011). In a study by Tatar et al. (2012), the effects of T. polium aerial parts extracts on oral glucose tolerance tests and pancreas histology in streptozocin-induced diabetic rats, were investigated. The histopathological investigation, along with the biochemical evaluations, indicated that treatment of diabetic rats with T. polium resulted in the regeneration of the pancreatic islets and reduction of the severity of streptozotocin-diabetic pancreases. The authors concluded that the extract of the aerial parts of T. polium probably stimulates pancreas repair and may be clinically beneficial as an agent to restore or maintain pancreas tissue after injury. Mousavi et al. (2012) investigated the effects of T. polium ethyl acetate extract on serum, liver, and muscle triglyceride content of sucrose-induced insulin resistance in rats. The treatment of rats with T. polium ethyl acetate extract resulted in a dose-dependent reduction in serum, liver, and muscle triglyceride (TG) and liver glycogen content levels and serum insulin. They concluded that these effects might be attributed, in part, to the hypolipidemic, hepatoprotective, and antioxidant activity of T. polium flavonoids. In a study performed by Mousavi et al. (2015), the beneficial effects of T. polium and metformin on diabetes-induced memory impairments and brain tissue oxidative damage in rats were evaluated. Results indicated that treatment with decoctions of T. polium for six weeks relieves the deleterious effects of diabetes on learning and memory. Phenolic-rich T. polium reduces oxidative damage to the hippocampus and cerebral cortex synapses, thus correcting learning and memory deficits in diabetes patients.
Regarding the significant effects of T. polium on β-cell regeneration and insulin secretion in animal models of type 1 diabetes, Tabatabaie and Yazdanparast (2017) investigated the molecular mechanism involved in the β-cell regeneration. Their results indicated that the antidiabetic effect of T. polium is strongly mediated via the antioxidant defense system and the Pdx1 expression in the JNK pathway of the streptozotocin (STZ)-induced diabetic rats pancreas. Recently, in a study by Amrae et al. (2020), the effects of the different fractions of T. polium on the aldose reductase enzyme (AR) activity as a strategy to reduce retinopathy were investigated. Results indicated that all fractions were found to inhibit lens AR activity. Among the different fractions and crude extract, the ethyl acetate fraction had the highest AR inhibitory activity (IC 50 = 3.67 μg/ml). In addition, the results showed noncompetitive inhibition of AR by the ethyl acetate fraction of T. polium.

Anticancer activities
In a study by Stankovic et al. (2011), antiproliferative and proapoptotic activity of methanolic extracts from different Teucrium species and its effect on the prooxidant/antioxidant status in HCT-116 cells were investigated. MTT assay indicated that all species, including T. polium, significantly reduced cell viability in a dose-dependent manner, with very low IC 50 values. Among all species, the methanol extracts from T. polium had a moderate cytotoxic effect after 24 hr (IC 50 = 77.83±0.4 µg/ml) and 72 hr (IC 50 = 253.39±1.61 µg/ml) of exposure. Also, the methanolic extracts of T. polium had a remarkable effect on superoxide anion radical (O 2 − ) and nitrite (NO 2 − ) production in HCT-116 cell line after 24 and 72 hr of exposure. Their results indicated that these effects are attributed to the phenolic and flavonoids compound contents of the plants. Anticancer activity of T. polium on hepatocellular carcinogenic rats was studied by Movahed et al. (2014). The results showed that T. polium suppresses liver cancer development and this may be due to the high levels of flavonoids and antioxidant compounds in the plant.

Antibacterial, antiviral, antifungal, and antileishmanial activities
Essential oils isolated from Teucrium species, including T. polium, were investigated for antiphytoviral activity; this species showed moderate reduced Cucumber Mosaic Virus (CMV) infections (41.4%) (Bezić et al., 2011). In another study, Vahdani et al. (2011) showed that T. polium has mild antimicrobial activity on microorganisms. Djabou et al. (2013) identified that Corsican Teucrium essential oils have the potential to be used as food preservatives and to prevent the growth of nosocomial bacteria. In another study, the antibacterial effect of methanolic extracts of some Lamiaceae species, including T. polium against Escherichia coli and Staphylococcus aureus, has been tested by agar disk diffusion and micro-dilution assays. Results showed that T. polium extract has low antibacterial activity (Khaled-Khodja et al., 2014). The essential oils of two species, T. polium subsp. aurum and T. polium subsp. polium were tested for antibacterial effects against two nosocomial bacteria, and the results showed that both species had high antibacterial properties, especially against Tribolium castaneum and Callosobruchus maculatus. Also, the results from their study suggest that sesquiterpene-rich essential oils from T. polium subsp. capitatum (L.) had insecticidal activity and could be used as a potential control agent for stored-product insects (Khani and Heydarian, 2014). Antimicrobial effects of essential oil, ethanol, and aqueous extracts of T. polium L. were tested against 13 microorganisms. The results indicated that essential oil possessed the highest antimicrobial activity and was most effective against Proteus mirabilis, S. aureus, and Citrobacter freundei. Also, essential oils and ethanolic extracts showed high antifungal power against Microsporum canis, Scopulariopsis brevicaulis, and Trichophyton rubrum (inhibition percentage 18.94 to 100%). None of the samples had antifungal activity against Aspergillus fumigatus (Othman et al., 2017). Two years later, Ravan et al. (2019) reported that essential oil from T. polium could be used as a potential agent to control aphid. They showed that exposure to sublethal concentrations of essential oil caused a reduction in the intrinsic rate of natural increase (r m value) because it decreased the adult female longevity and fertility of surviving aphids. Simultaneously, results from a study by Ebadollahi and Taghinezhad (2019) on essential oils of T. polium showed high insecticidal efficiency against red flour beetle, and toxicity was increased with increasing exposure time and the amount of sublethal concentration.
In another study, essential oil from T. polium was evaluated for antileishmanial activity. The results illustrated that essential oil has potent inhibitory activity against two promastigote forms L. major and L. infantum, even its antileishmanial activity was higher than amphotericin B (control positive); the cytotoxicity on macrophage cells was low (Essid et al., 2015).

Effect on memory enhancement
In a review study, Williams et al. (2011) reported that extract from the aerial parts of T. polium is anti-amnesic in vivo and inhibits Acetylcholinesterase (AChE) in vitro. In another study, it was stated that this plant possesses a protective effect against memory impairment in diabetes (Hasanein and Shahidi 2012). Ali et al. (2013) indicated that aerial parts of T. polium improve mental performance and focus. AChE inhibitory activities of the ethanolic extracts of 26 medicinal plants of the Lamiaceae family, including T. polium, were investigated by Vladimir-Knežević et al. (2014). The results of this study showed that some of these species, including T. polium at 1 mg/ml indicated strong inhibitory activity against AChE. Also, Ahmadian-Attar et al. (2015) reported that in traditional medicine, the decoction of the aerial part of this plant is prescribed orally due to its anti-depressant properties. Mousavi et al. (2015) reported that the hydroalcoholic extract of T. polium inhibit diabetes-induced memory deficits in rats. In another study, it was reported that T. polium reduced ovariectomized (OVX)induced neurodegenerative alterations in entorhinal cortex-hippocamp circuitry and facilitated neuronal survival by modulating neurotransmitters' activity and network plasticity (Simonyan and Chavushyan 2016). Lobbens et al. (2017) reported that in Anatolia, this plant is used in traditional medicine to enhance memory.

Anti-ischemic and antiseizure effects
In a research by Khoshnood-Mansoorkhani et al. (2010), protective effects of T. polium ethanolic aqueous extracts and related fractions on seizures induced by pentylenetetrazole (PTZ) and maximal electroshock stimulation (MES) have been investigated. Their results showed that aqueous extract of T. polium and a related n-butanol fraction (ED 50 =12.6 mg/kg body weight) have antiseizure effects comparing to control groups and ethanolic extract. Authors believed that high levels of flavonoids in the aqueous extract may be the reason for this difference.
Regarding the deleterious effect of oxidative stress on myocardial ischemiareperfusion (I/R), Mahmoudabady et al. (2018) investigated the effects of the T. polium on I/R injuries in the isolated rat heart. Their results indicated that pretreatment with T. polium increased thiol (SH) groups, superoxide dismutase (SOD), and catalase (CAT) activities but decreased the lactate dehydrogenase (LDH), creatine kinase (CK) activity, and TBARS level; therefore, it can be concluded that this plant has a cardioprotective effect against oxidative stress during I/R injury.

Cardiovascular effects
Cardiovascular effects of T. polium extract in rabbits were investigated by Niazmand et al. (2011). Their study showed that aqueous-ethanol extract had no effect on heart rate but showed a positive inotropic on the heart. In addition, the extract of T. polium indicated hypotensive effect. The reason for these observations is the inotropic effect of the extract. Results from a study about the synergic effect of T. polium and tranilast on human umbilical vein endothelial cells (HUVECs) by Sheikhbahaei et al., 2018 showed that this plant in a dose-and timedependent manner, alone or in combination reduced the viability of HUVECs. Mahmoudabady et al. (2014) indicated that T. polium extract could help to prevent high blood pressure induced by Angiotensin II (Ang II) pathway activation. In another study, in vitro antiatherothrombotic effects of extracts from three species, including T. polium, were studied. The data showed prolonged coagulation time in a concentrationdependent manner following administration of aqueous extract and polysaccharide extract from T. polium implying a potential antithrombotic property for this plant (Nor et al., 2019).

Hepatoprotective activity
In a study by Fiorentino et al. (2011), the hepatoprotective activity of seven neoclerodanes (teupolins VI-XII) and eleven known compounds isolated and purified from a polar extract of T. polium leaves against the human hepatoblastoma cancer cell line HepG2 was evaluated. Data from a study by Forouzandeh et al. (2013), suggest that T. polium aqueous-ethanol extract has a protective effect on acetaminophen-induced hepatotoxicity in mice because their findings showed a decrease in the serum liver enzyme activities (alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP)) and bilirubin concentrations. Moreover, the liver morphology and histopathology findings confirmed the protective activity of this extract against the acetaminophen-induced liver damage as shown by the reversal of centrilobular necrosis, fatty changes (steatosis) and scattered lymphocytes infiltrate in hepatic parenchyma by T. polium administration. Also, Rafieian-Kopaei et al. (2014) reported that T. polium has a protective effect on hepatotoxicity. At the same time , Jadeja et al. (2014) reported that alkaloid from T. polium extract has hepatotoxicity. Jadeja et al. (2014) reported that ethyl acetate fraction of T. polium could be used for the treatment of nonalcoholic steatohepatitis. Protective activity of total polyphenols from Genista quadriflora Munby and T. polium geyrii Maire in acetaminopheninduced hepatotoxicity in rats, was investigated by Baali et al. (2016). Their study results showed that polyphenolic extracts of T. polium and G. quadriflora had hepatoprotective activity and reduced transaminase leakage. At the same time, in a review study, Pour et al. (2019) reported that T. polium is useful for the hardness of the spleen, splenitis, black jaundice, and dropsy (ascites). Alizadeh et al. (2011) indicated that honey of T. polium could assist wound healing and tensile strength in rat skin wounds. Also, Hosseinkhani et al. (2017), based on the belief of traditional Persian medicine, showed that T. polium aerial part could be used for various wounds. The results of studies conducted by Meguellati et al. (2019) identified that the extract of this plant has the property of healing skin wounds in rats, the use of callus obtained from the aerial parts of T. polium improved the wound after 11 days which by far exceeded the threshold marked by the reference. Khadige et al. (2016) in a triple-blind placebo-controlled clinical trial, investigated the effect of T. polium on reducing menstrual bleeding. The result of their study showed that T. polium significantly decreases duration and amount of menstrual bleeding in the 1st and the 2nd menstruation cycles after treatment. In another study, the effect of T. Polium extract administration on spermatogenesis and testicular structure in diabetic rats induced with streptozotocin was investigated. Results showed that the hydroalcoholic extract of T. polium has a protective effect on diabetes-induced testicular damage and serum testosterone concentration. This effect was related to antioxidant and antidiabetic properties of the hydroalcoholic extract of T. polium (Salimnejad et al., 2017). Khadige et al. (2016) showed that T. polium was as effective in reducing the pain severity in primary dysmenorrhea as mefenamic acid. In a review article, Uritu et al. (2018) reported that T. polium might be effective in some type of pain, for example, visceral pain (Abdollahi et al., 2003), menstrual cycle pain, pain-related behavior in the diabetic rat formalin test (100 or 200 mg/kg body weight) (Baluchnejadmojarad et al., 2005). In addition, the ethanolic extract of the plant in dose of 500 mg/kg bodyweight inhibited carrageenan-induced inflammation and reduced granuloma formation (Tariq et al., 1989).

Hypolipidaemic and anti-inflammatory effects
The effects of a polyherbal mixture, including T. polium, were tested on biochemical parameters in diabetic rats. The results showed that this polyherbal mixture has beneficial effects on blood glucose and lipid profile (Niazmand et al., 2017). The finding of the study by Safaeian et al. (2018) indicated that various fractions derived from hydroalcoholic extract of T. polium had a strong antihyperlipidemic effect, but chloroform fraction had the highest hypolipidemic activity in a dose-dependent manner. In a study on the antiinflammatory effects of the plant, the essential oil from the aerial parts of T. polium ssp capitatum showed antiinflammatory activity, being able to inhibit LPS-induced NO production (Fitsiou and Pappa, 2019).
Cytotoxicity Dağ et al. (2014) indicated that T. polium has a potential hepatotoxic effect; however, physiological changes during pregnancy and postpartum periods may increase the severity of such toxicity that should be considered in the differential diagnosis. Kristen and Kreft in 2016 reported that many plant species of the genus Teucrium including T. polium, cause moderate liver damage in rodents. Furancontaining diterpenoids are toxic components of the extract, which after about a month can lead to cholestatic hepatitis. Al-Qahdi et al. (2019) showed that women should avoid taking T. polium plants during pregnancy because the plant can have very toxic effects on the early stage of the embryo. Lin et al. (2019) reported that T. polium from Greece induced liver injury. Recently, it was reported that T. polium might lead to liver injury (often with a cholestatic signature). The toxicity mechanism is related to furano neoclerodane diterpenoids, teucrin A and teuchmaedryn A, with too highly reactive epoxides considered as inducers of hepatocyte apoptosis (Vilas-Boas et al., 2020).

Discussion
Our T. polium literature search covering the years 2011 up to 2020 indicated that most of the studies focused on the antidiabetic and antioxidant activities of the various extracts of the plant. Due to the role of oxidative stress in many diseases on one hand and the high antidiabetic and antioxidant potency of the plants, on the other hand, it is predicted that the T. polium extracts and/or elucidated components of the extracts could be beneficial for the treatment of a wide range of diseases, pending further investigation to eliminate the cytotoxicity, if any, of the crude or partially purified fractions of T. polium.